Continuous and batch cultures of Escherichia coli KJ134 for succinic acid fermentation: metabolic flux distributions and production characteristics

Table 1 Gene modifications to E. coli C to obtain the succinate-producing E. coli KJ134[14, 24]

Enzyme	Modification	Abbreviation
2-ketobutyrate formate lyase	Inactivation	∆ tdcE
Acetate kinase	Inactivation	∆ ackA
Alcohol dehydrogenase	Inactivation	∆ adhE
Aspartate aminotransferase	Inactivation	∆ aspC
Citrate lyase	Inactivation	∆ citF
Formate transporter	Inactivation	∆ focA
Lactate dehydrogenase	Inactivation	∆ ldhA
Methylglyoxal synthase	Inactivation	∆ mgsA
NAD⁺-linked malic enzyme	Inactivation	∆ sfcA
PEP carboxikinase	Overexpression	∆ pck
Phosphotransacetylase	Inactivation	∆ pta-ackA
Pyruvate formate lyase	Inactivation	∆ pflB
Pyruvate oxidase	Inactivation	∆ poxB
Threonine decarboxylase	Inactivation	∆ tdcD

ISSN: 1475-2859