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Table 5 Primers used for construction of expression cassette acoA-nk-T7

From: Cloning and enhancing production of a detergent- and organic-solvent-resistant nattokinase from Bacillus subtilis VTCC-DVN-12-01 by using an eight-protease-gene-deficient Bacillus subtilis WB800

Primer

Sequence 5′→3′

PCR product

NkF

GC GAATTC GC GTGAGAAGCAAAAAATTG

nk

NkR

GC CTCGAG TTGTGCAGCTGCTTGTAC

 

acoA F

GC GAATTC TCAGTCAAACGATGCAG

Fragment A

acoA R

AGC GCT CGC AGC CGC CGG TCC TGC

 

acoA-NkF

GAC CGG CGG CTG CGA GTG CTg tga gaa gca aaa aat tgt g

Fragment B

T7R

GC GGATCC CAA AAA ACC CCT CAA GA

Â