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Table 1 Purification steps of the nattokinase from B. subtilis WB800/pANk

From: Cloning and enhancing production of a detergent- and organic-solvent-resistant nattokinase from Bacillus subtilis VTCC-DVN-12-01 by using an eight-protease-gene-deficient Bacillus subtilis WB800

Step

Total activity (U)

Total protein (mg)

Specific activity (U/mg)

Purification factor

Yield (%)

Crude nattokinase

24

4.8

5.0

1.0

100

ProBondâ„¢ resin

13

0.8

16.25

3.25

54.17