Table 2 Comparison of the production yield and mannanase activity produced by different expression plasmids
Expression plasmid | The amount of total protein(mg/ml) | The amount of mannanase Man23(mg/ml) | Mannanase activity(U/mg) |
|---|---|---|---|
Wild-type | 8.0 ± 0.06 | 0.72 ± 0.02 | 188.5 ± 0.3 |
pHY-p43-man23 | 9.5 ± 0.05 | 8.1 ± 0.05 | 222.5 ± 0.2 |
pBPS-man23 | 8.3 ± 0.05 | 5.8 ± 0.03 | 195 ± 0.2 |