Table 2 List of plasmids and strains in this work

pET28a(+)

Kan^R; T7 promoter based E. coli expression vector

Novagen

pET28a-ACP

pET28a(+) derivative carrying M. purpureus ACP encoded DNA

This study

pET28a-ACPm

pET28a(+) derivative carrying M. purpureus ACPm encoded DNA

This study

pESC-ATX

Amp^R; URA3; P _GAL1 / _GAL10 -based yeast expression vector; commerial pESC-URA derivative carrying atX gene

Moriguchi et al., 2006

pPIC3.5 K

Amp^R G418^R; HIS4; P _AOX1 -based yeast expression vector

Invitrogen

pPICZ B

Zeocin^R ; P _AOX1 -based expression yeast vector

Invitrogen

pPIC3.5 K-NpgA

pPIC3.5 K derivative carrying A. nidulans npgA gene

This study

pPICZ B-NpgA-HIS₆

pPICZ B derivative carrying A. nidulans npgA gene

This study

pPICZ B-ATX

pPICZ B derivative carrying A. terreus atX gene

This study

pPICZ B-CT-SKL

pPICZ B derivative carrying M. purpureus pksCT gene a P. pastoris SKL sequence

This study

pPICZ B-CT

pPICZ B derivative carrying M. purpureus pksCT gene

This study

pPIC3.5 K-NpgA-SKL

pPIC3.5 K derivative carrying A. nidulans npgA gene and a P. pastoris SKL sequence

This study

Strains

Characteristic(s)

Reference

GS115

his4, AOX 1, AOX2

Invitrogen

GS115-NpgA-HIS₆

Zeocin^R; GS115 with plasmid pPICZ B-NpgA-HIS₆

This study

GS115-ATX

Zeocin^R; GS115 with plasmid pPICZ B-ATX

This study

GS115-NpgA

G418^R; GS115 with plasmid pPIC3.5 K-NpgA

This study

GS115-NpgA-ATX

Zeocin^R; G418^R; GS115-NpgA with plasmid pPICZ B-ATX

This study

GS115-3.5 K

G418^R; GS115 with pPIC3.5 K

This study

GS115-3.5 K-CT-SKL

Zeocin^R; G418^R; GS115-3.5 K with pPICZ B-CT-SKL

This study

GS115-NpgA-CT

Zeocin^R; G418^R; GS115-NpgA with plasmid pPICZ B-CT

This study

GS115-NpgA-SKL

G418^R; GS115 with plasmid pPIC3.5 K-NpgA-SKL

This study

GS115-NpgA-CT-SKL

Zeocin^R; G418^R; GS115-NpgA with plasmid pPICZ B-CT-SKL

This study

GS115-NpgA-SKL-CT-SKL

Zeocin^R; G418^R; GS115-NpgA-SKL with plasmid pPICZ B-CT-SKL

This study

E. coli TOP10

F’ [lacIq, Tn10(TetR)] mcr A φ80lac ZΔM15 Δlac X74 deo R rec A1

Invitrogen

E. coli BL21

F^–ompT hsdS _B (r_B^– m_B^–) gal dcm (DE3)

Invitrogen

BL21-ACP

Kan^R; BL21 with plasmid pET28a-ACP

This study

BL21-ACPm

Kan^R; BL21 with plasmid pET28a-ACPm

This study