Engineered fungal polyketide biosynthesis in Pichia pastoris: a potential excellent host for polyketide production

Table 1 Primers used in this study

Primer	Sequence (5′ to 3′)^a
NpgA-HIS6-F	GGACCGCGG CCACCATGGTGGACTACAAGGATGACGATGACAAGGGT
NpgA-HIS6-F	GGAGGTGGATCTGTGCAAGACACATCAAGCGCAAG
NpgA-HIS6-R	ATAAGAATGCGGCCGC GCGGATAGGCAATTACACACC
5AOX1	GACTGGTTCCAATTGACAAGC
3AOX1	GCAAATGGCATTCTGACATCC
AcpF	CATGGCTAGC GATGGTTCGTCTACTGCGGT
AcpR	CCGCTCGAG TGCTGATTTTGGGAGGATGGAACC
Mutant5	TGGAATCGATGCGCTGATGAGCATG
Mutant3	CATGCTCATCAGCGCATCGATTCCA
NpgAF	GGAATTCTACGTA ACCATGGTGCAAGACACATCAAGCGCAAG
NpgAR1	ATGGGTACAGATCCTCTTCTGAGATGAGTTTTTGTTCGGATAGGCAATTACACACCCCAGTC
NpgAR2	ATAAGAATGCGGCCGC TTAAGCGTAATCTGGAACATCGTATGGGTACAGATCCTCTTCTGAGATG
AtxF	CGGGAATTCACCATGGAGGTACATGGAGATGAAGTG
AtxR	ATAAGAATGCGGCCGCCTTTCCCATCTTTTCCAAAAACCAT
BstpF	AACTGCAGAAGAATTCGGTTACCTGTACGTGGAAAAGGCTG
BstpR	AACTGCAGAAGGTAACCCACCTTGGGAGGCGGCTG
CTAF	TCCCCGCGG CATATGCAATTTATCCGGTCTTG
CTAR	ATAAGAATGCGGCCGC TTACAACTTAGAATCTAGAAATCCCATGGTCTTCC
CTBF	TCCCCGCGG TCGCGAGTACGAAGGTTTATC
CTBR	GGAATTCCATATG ATCTCCCGCATCTTC
CTCF	CGCCGCGG GACATGGCTCATCATCACCATCACCATGGTGGAGGTGGATCTATGATTGACTCAACTTCGCACTC
CTCR	CTAGCCTCGCGA TCACACCAAATACC
Citrinin-F2	CCATCGATTCG CTGATGAGCATGGAG-3
Citrinin-R2	GGGAATTCGCGGCCGC ATGATCTCTTGGCGCCCTG
NpgASR1	ATGGGTACAGATCCTCTTCTGAGATGAGTTTTTGTTCGGATAGGCAATTACACACCCCAGTC
NpgASR2	ATAAGAATGCGGCCGC TTACAACTTAGAAGCGTAATCTGGAACATCGTATGGGTACAGAT CCTCTTCTGAGATG
1977 F	TTCCTCAGCCCTACTGGTCAAT
4088R	GCTGGGATGCGTCTTGATAACC

ISSN: 1475-2859