Figure 6

Fluorescent assay of ACP and ACPm that were fluorescently labelled. The KODAK In-vivo multispectral imaging systerm F was used for scanning. (A) Image parameter of 480 nm laser and 535 nm emission filter; (B) Image parameter of 580 nm laser and 670 nm emission filter; (C) Image of the same SDS-PAGE gel stained with Coomassie brilliant blue R-250. Lane 1: In vitro reaction containing Bodipy FL-CoA, PPtase and ACPm; Lane 2: In vitro reaction containing Bodipy-CoA, PPtase and ACP; Lane 3: In vitro reaction containing Alexa Fluor 647-CoA, PPtase and ACPm; Lane 4: In vitro reaction containing Alexa Fluor 647-CoA, PPtase and ACP. Protein preparation were described in Methods.