Figure 2
The EI-MS identification of product 6-MSA by GS115-NpgA-ATX and transcription analysis of npgA and atX . (A) EI-MS analysis of extract from GS115-NpgA-ATX. m/z, mass-to-charge ratio; (B) The 1HNMR analysis of extract from GS115-NpgA-ATX. Samples preparation were in the Section 6-MSA extraction and identification in Methods; (C) Lane 1–3: PCR using cDNA of wild type GS115 and primers 5AOX1/3AOX1, NpgA-F/R1 and BstpF/AtxR, respectively; Lane 4–6: PCR using GS115-NpgA-ATX cDNA and primers 5AOX1/3AOX1, NpgAF/ NpgAR1 and BstpF/AtxR, respectively; M: DNA marker Hind III. Gene transcription induced by 0.5% methanol for 24 h.