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Table 2 Effects of PDR5 5′ UTR on Cdr1p and Cdr2p expression

From: Small, synthetic, GC-rich mRNA stem-loop modules 5′ proximal to the AUG start-codon predictably tune gene expression in yeast

AD/+

PDR5 5′ UTR++

MICFLC(mg/l)

CDR1A

PacI

200

SfiI

50

SfiI(−4)

50

CDR2A +++

PacI

120

SfiI

20

CDR2B +++

PacI

240

 

SfiI

30

  1. + AD strains containing the indicated ORFs followed by the PGK1ter and URA3 marker.
  2. ++ Nucleotide changes to the PDR5 5′ UTR just upstream of the ATG start codon (SfiI(−4) = CDR1-construct #1).
  3. +++ The two alleles of CDR2 from C. albicans ATCC 10261.
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Microbial Cell Factories

ISSN: 1475-2859

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