Small, synthetic, GC-rich mRNA stem-loop modules 5′ proximal to the AUG start-codon predictably tune gene expression in yeast

Table 1 Effects of PDR5 5′ UTR and transcription terminator on Pdr5p expression in yeast strains AD and AD-sec6-4

⁺∆PDR5 (top) = AD1-8u^-. The strains underneath contain either the entire wt PDR5 gene (WT) or were modified in their 5′ UTRs just upstream of the ATG start codon to create the restriction sites indicated in the neighbouring column. The strains in the bottom three rows are identical to the strains above except that the PGK1ter and URA3 marker were placed between the ORF and the PDR5 terminator.
nd = not determined, this strain was not constructed.

ISSN: 1475-2859