Figure 4
Cdr1p expression levels decrease exponentially with an increase in the number of GC-pairs in stems containing only GC-pairs 5′ proximal to the AUG start-codon. A. The relationship between MICFLC values of Cdr1p-expressing strains and the number of GC-pairs in stems containing only GC-pairs. Black squares represent MICFLC values and black triangles represent the lnMICFLC values plotted against the number of GC-pairs in stem-loop constructs. The two black lines represent the trend lines for the best fit of these two data sets (‘error’ bars indicate the possible range of MICFLC values of individual constructs; see text for further details). The horizontal black dashed lines mark the MICFLCmax values of wild type Cdr1p-expressing cells. The green dashed trend line is the trend line that was created with the calculated MICFLC values for stem-loop constructs containing only GC-pairs using the formula shown underneath. B. The relationship between MICFLC values of Cdr1p-expressing strains and the calculated ∆G values for stems containing only GC-pairs. The same symbols as in A were used. The dashed grey lines are the trend lines for the best fit including the data point for the construct containing 2 GC-pairs, while the two black lines represent the trend lines for the best fit of the data sets excluding that data point. The formula that can predict the MICFLC values for stem-loop constructs containing only GC-pairs using their calculated ∆G values is shown underneath. All MICFLC values are the values for three independent transformants and did not vary by more than one dilution.