Effect of culture medium, host strain and oxygen transfer on recombinant Fab antibody fragment yield and leakage to medium in shaken E. coli cultures

Table 1 Cell density, pH and percentage of extracellular Fab in different media in 24dwp

Cell density was determined by optical density measurements at 600 nm (OD₆₀₀), and pH was measured from culture supernatant at room temperature. Mean and standard deviation of two independent replicate experiments are shown. In the fed-batch-like cultures (EnBase) OD₆₀₀ is shown at 24 h (6 h from induction) and 42 h (25 h from induction). In ZYM-5052 autoinduction cultures OD₆₀₀ is shown at 19 h and 42 h from cultivation start. In Super Broth cultures OD₆₀₀ was measured after 19 h incubation in the inducing medium. pH was measured at the end of cultivation. The percentage of extracellular Fab represents the situation at the end of cultivation.

ISSN: 1475-2859