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Figure 4 | Microbial Cell Factories

Figure 4

From: Effect of culture medium, host strain and oxygen transfer on recombinant Fab antibody fragment yield and leakage to medium in shaken E. coli cultures

Figure 4

Fab expression in E. coli BL21(DE3) in shake flask cultures. Yields in mg per liter of culture for Fab fragments F1 (a), F16 (b), F32 (c) and 1B10 (d) expressed in E. coli BL21(DE3) in Ultra Yield shake flasks at two different shaking speeds (150 rpm and 250 rpm), as measured by antigen-binding ELISA from cell lysate (periplasmic fraction; in black) and broth supernatant (medium fraction; in grey). All fragments were expressed in the fed-batch-like EnBase medium (EB) and ZYM-5052 autoinduction medium (ZYM). Samples were drawn for analysis at 24 h after induction in EB, and 24 h and 41 h after cultivation start in ZYM. Standard deviations for the ELISA analysis are shown in Additional file 2.

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Microbial Cell Factories

ISSN: 1475-2859

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