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Figure 3 | Microbial Cell Factories

Figure 3

From: Effect of culture medium, host strain and oxygen transfer on recombinant Fab antibody fragment yield and leakage to medium in shaken E. coli cultures

Figure 3

Fab expression in E. coli RV308 in shake flask cultures. Yields in mg per liter of culture for Fab fragments F1 (a), F16 (b) and F32 (c) expressed in E. coli RV308 in the fed-batch-like EnBase medium in Ultra Yield shake flasks at two different shaking speeds (150 rpm and 250 rpm), as measured by antigen-binding ELISA from cell lysate (periplasmic fraction; in black) and broth supernatant (medium fraction; in grey). Samples were drawn 24 after induction. A-C on the horizontal axis refer to independent replicate experiments. Standard deviations for the ELISA analysis are shown in Additional file 2.

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