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Figure 1 | Microbial Cell Factories

Figure 1

From: Effect of culture medium, host strain and oxygen transfer on recombinant Fab antibody fragment yield and leakage to medium in shaken E. coli cultures

Figure 1

Yields of Fab fragments in mg per liter of culture in different media. Quantities of Fab fragments F1, F16, F32 and 1B10 were measured by antigen-binding ELISA from cell lysate (periplasmic fraction; in black) and broth supernatant (medium fraction; in grey). All fragments were expressed in E. coli RV308 and BL21 in 24 deep well plates. Samples were drawn for analysis at 24 h after induction in the fed-batch-like EnBase medium (EB), 19 h after induction in Super Broth (SB), and 19 h and 42 h after cultivation start in ZYM-5052 autoinduction medium (ZYM; 19 h data not shown, yields at 19 h were lower than at 42 h). The mean values of two independent replicate cultivations are shown.

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Microbial Cell Factories

ISSN: 1475-2859

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