Table 2 Strains and plasmids used in this study
From: Metabolic engineering of Bacillus subtilis for growth on overflow metabolites
Strain | Genotype | Reference |
|---|---|---|
B. subtilis 6051HGW | Wild type | [24] |
B. licheniformis MW3 | ΔhsdR1, ΔhsdR2 | [23] |
B. subtilis ACE | ΔamyE::aceBA | This study |
B. subtilis ACEProm1 | ΔamyE::aceBA | This study |
B. subtilis ΔamyE | ΔamyE::EryR | This study |
B. subtilis acoAamyE | ΔamyE::EryR ΔsacA::acoAamyESSS | This study |
B. subtilis ACEamyE | ΔamyE::aceBAProm1SSE ΔsacA::acoAamyESSS | This study |
E. coli DH10B | F-mcrA,(mrr-hsdRMS-mcrBC)’80lacZ, M15, lacX74, recA1 araD139, (ara leu) 7697 | [25] |
Plasmid | Function | Reference |
pBGAB | Integration of genes into the amyE locus with NeoR | [26] |
pACEBA | aceBA chromosomal integration into the amyE locus without promoter | This study |
pMTL500 | Source of ermB gene for erythromycin resistance | [27] |
pAMYSSE | Integration of genes into the amyE locus with EryR | This study |
pProm1 | Integrating of the native aceBA promoter and the gene encoding the hypothetical protein | This study |
pJK168 | Integration of genes into the sacA-locus | J. Kumpfmüller (unpublished results) |
pMJS2 | Source of the P acoA -amyE construct | [13] |
pSacAmyE | Integration of the amyE gene under P acoA control into the sacA-locus | This study |