Table 1 Oligonucleotide primers used in this study
From: Metabolic engineering of Bacillus subtilis for growth on overflow metabolites
Name | Sequence 5’ → 3’ | Usage |
|---|---|---|
aceBAfor | GATCGTCTAGAGTCTCCCTACCCCTTTACAT | Forward primer for aceBA operon |
aceBArev | GATCGGATCCAAGACAACCGGCCGGCCAAA | Reverse primer for aceBA operon |
Prom1-for | GAAAATGGCTAAAATTGGTTATGCACGACTCTACGAAT | Forward primer for integration of native promoter |
Prom1-rev | CGCAACGCGGGGAGGCAGACAAGGTATAGGGCGGGG ACCAAAATCGAACAGGCTTGC | Reverse primer for integration of native promoter |
SLIC-Ery-for | TTATGCACGACTCTAGCCTGCAGGTCGACTGATAAGACGGTTCGTGTTCG | Forward primer for EryR cassette |
SLIC-Ery-rev | TAATTAGACCGGTACCCGGGGATCCTCTAGCTCTTTAGCTCCTTGGAAGC | Forward primer for EryR cassette |
aceB-for | CTGCCGCCGATCAGCACATGC | Forward primer for mRNA-standard of aceB |
aceB-rev | CCCGCAAATTAACCTGGC | Reverse primer for mRNA-standard of aceB |
aceB-rev-T7 | TAATACGACTCACTATAGGGCCCGCAAATTAACCTGGC | Reverse primer for mRNA-standard of aceB with T7-promoter |