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Table 2 Fatty acid composition in cellular and extracellular lipids of Polh-1292-sp opai-d12-16 in fermentations with YNBD-SO medium

From: Genetic engineering of Yarrowia lipolytica for enhanced production of trans-10, cis-12 conjugated linoleic acid

Fatty acid

Polh-1292-spo pai- d12-16

Cell - 0 h

Cell - 38.5 h

Medium - 0 h

Medium – 38.5 h

16:0

12.2 ± 0.6

9.3 ± 0.4

9.9 ± 0.5

11.8 ± 0.6

16:1

7.9 ± 0.8

0.9 ± 0.1

ND

ND

16:2

1.7 ± 0.2

ND

ND

ND

17:1

0.3 ± 0.0

ND

ND

ND

18:0

0.9 ± 0.1

3.6 ± 0.2

3.5 ± 0.1

6.3 ± 0.3

18:1

15.7 ± 1.0

20.2 ± 0.7

19.4 ± 0.9

23.0 ± 1.2

18:2

60.6 ± 2.6

14.6 ± 0.6

57.5 ± 2.2

22.5 ± 0.7

18:3

ND

3.4 ± 0.1

8.0 ± 0.5

2.1 ± 0.3

10 t, 12c-CLA

0.3 ± 0.0

44.3 ± 2.1

ND

30.4 ± 1.1

  1. Values are presented as % (w/w) of the total fatty acid levels in Polh-1292-spopai-d12-16. Other fatty acids were C12:0, C14:0, C20:0, C20:1, C20:2, C22:0 and C24:0 (not shown). The means ± SD were obtained from three independent experiments. ND, not detected.