Figure 2

Transcript and protein levels of recombinant PAI and CLA yield in different Y. lipolytica strains. For comparative analysis of opai transcription and protein expression levels, five representative transformants with an average CLA yield in each series were selected, along with the best-performing transformant, Polh-pINA1292-spopai-d12-16. The Y. lipolytica strains were harvested after 72 h cultivation in YPD medium. Transcript levels were determined by real-time PCR and protein levels were analyzed by western blot analysis using specific polyclonal antibodies. The control used was the strain transformed with the empty vector pINA1312. The standard deviations were < 5% of the values shown.