Figure 1

Relative copy numbers of integrated expression cassettes in Y. lipolytica multi-copy transformants. Real-time PCR was used to estimate the copy numbers of the integrated expression cassettes among 12 Polh-1292-spopai-d12 transformants. Y. lipolytica Polg was used as a control organism with a single copy of both the ura3 and suc2 target sequences. As ura3, opai and d12 coexisted in expression cassettes, the copy numbers of three genes were considered to be equal. Error bars represent standard deviations from biological triplicates.