Kinetics of lactate synthesis by strain LA20 (pZSglpKglpD) in shake flasks containing minimal medium with 40 g/L of glycerol. Data for concentration of cells (∎), glycerol (♦), lactate (▲) and acetate (●), along with lactate yield (∆), are shown. Coefficients of variation (i.e. standard deviations/average × 100) were below 5% in all cases. The inset shows a high-resolution data set for late stages of cultivation: symbols and axis titles are as specified for the main figure.