Table 1 Overview of the energy charge obtained by the different investigated methods
From: Methodological approaches to help unravel the intracellular metabolome of Bacillus subtilis
Sampling method | Energy charge |
|---|---|
5 min centrifugation, without washing (cooled 60% EtOH) | 0.36 ± 0.076 |
Filtration, including washing (cooled H 2 O) | 0.23 ± 0.140 |
Filtration, including washing (cooled MeOH/H 2 O/CHCl 3 -mixture) | 0.28 ± 0.129 |
Filtration, including washing (boiling 60% EtOH) | 0.19 ± 0.055 |
Filtration, including washing (cooled 60% EtOH) | 0.43 ± 0.077 |
Filtration, including washing (cooled 60% EtOH ⇨ CHCl 3 ) | 0.41 ± 0.069 |
Filtration, including washing (cooled 60% EtOH ⇨ H 2 O) | 0.43 ± 0.099 |
Filtration, without washing (cooled 60% EtOH ⇨ H 2 O) | 0.71 ± 0.040 |
N 2 cooling, filtration, without washing (cooled 60% EtOH ⇨ ACN) | 0.80 ± 0.016 |
N 2 cooling, filtration, without washing (cooled 60% EtOH ⇨ H 2 O) | 0.81 ± 0.033 |