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Figure 3 | Microbial Cell Factories

Figure 3

From: Methodological approaches to help unravel the intracellular metabolome of Bacillus subtilis

Figure 3

Comparison of the detected relative metabolite amounts via GC-MS measurement. Comparison of the detected relative metabolite amounts after several tested extraction techniques related to cooled 60% (w/v) methanol (black line at 100%). Boiling water (yellow), boiling (red) so as cooled (green) 60% (w/v) ethanol, 1 M formic acid (dark blue), 100% acetonitrile (light blue) and methanol/water/chloroform (4:2:4) extraction (purple). Measurements were performed by GC-MS and for comparisons of the extraction potential, 60% (w/v) methanol extractions were carried out in parallel for each tested extraction solution. C5-sugar-P and C6-sugar-P refers to sugar-phosphates containing 5 or 6 carbon atoms respective. The y-axis is log10 scaled.

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