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Figure 6 | Microbial Cell Factories

Figure 6

From: A single mutation in the core domain of the lac repressor reduces leakiness

Figure 6

LacIWFshows a wider dynamic range than WT. GFP expression levels measured on 200 μL of cell culture bearing LacI (blue, left axis) or LacIWF (red, right axis) after 24 h incubation at 37°C with different IPTG concentrations. For each variant, three independent repeats were corrected for cell density. The plot shows averaged values and standard deviation for each point. Curves have been drawn merely to guide the eye. The two datasets are plotted on different axes to show the difference in dynamic range between LacI and LacIWF.

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