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Figure 3 | Microbial Cell Factories

Figure 3

From: A single mutation in the core domain of the lac repressor reduces leakiness

Figure 3

GFP production kinetics for LacI and LacIWF. Cells transformed with either pLIGFP (blue/cyan) or pLIGFP_W220F (red/brown) were grown at 37°C in the presence (solid symbols) or absence (open symbols) of IPTG. At regular intervals from the time of induction (time 0), the cell culture was sampled for FACS (panel A) or cell extract (panel B) analysis. The original flow cytometry histograms are provided in Additional file 1: Figure S3.

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