Figure 2
From: A single mutation in the core domain of the lac repressor reduces leakiness
LacIWFaffords higher GFP expression levels and lower leakiness. The expression of GFP was quantified by flow cytometry of E. coli cells carrying pLIGFP with the indicated mutations at position 220. Cells were incubated overnight at 37°C and the geometric mean of fluorescence measurements in the absence (blue bars) and presence (orange bars) of IPTG (0.01 mM) is displayed as arbitrary units (a.u.). To allow the comparison of data acquired on different days, fluorescence values have been normalised against WT (all mutants were tested under the same conditions and WT was measured each time). The original flow cytometry histograms are provided in Additional file 1: Figure S2.