Table 1 Strains and plasmid used in this study
Strains and plasmids | Relevant features | Reference |
|---|---|---|
E. coli strain | ||
Novablue | endA1 hsdR17(r K12 -m K12 +) supE44 thi-I gyrA96 relA1 lac recA1/F’[proAB+lacIq ZΔM15::Tn10(Tetr)] | Novagen |
S. cerevisiae yeast strains | ||
MT8-1 | MATa ade leu2 his3 ura3 trp1 | [18] |
MT8-1/cocδBEC1 | MATa ade leu2 his3 ura3, expressing β-glucosidase, endoglucanase and cellobiohydrolase genes on its cell surface | [19] |
MT8-1/δSWO | MATa ade leu2 his3 trp1, expressing SWOI genes from T. reesei on its cell surface | This study |
MT8-1/δELPAO | MATa ade leu2 his3 trp1, expressing AoelpI genes from A. oryzae on its cell surface | This study |
MT8-1/cocδBEC1/δSWO | MATa ade leu2 his3, expressing β-glucosidase, endoglucanase, cellobiohydrolase, and SWOI genes on its cell surface | This study |
MT8-1/cocδBEC1/δELPAO | MATa ade leu2 his3, expressing β-glucosidase, endoglucanase, cellobiohydrolase, and AoelpI genes on its cell surface | This study |
Plasmids | ||
pδU-PGAGSWO | URA3, expression of SWOI by δ-integration | This study |
pδU-PGAGELPAO | URA3, expression of AoelpI by δ-integration | This study |