Table 2 Primers used in this study
Genes and primers | Sequence (5′-3′) |
|---|---|
rimP homologues relevant primers | Â |
Lrimp-SC-F | CCCAAGCTTGGCCAGCCGGTCCTCCAGTT |
Lrimp-SC-R | GCTCTAGAGGTGGTGCTCATCCGGGTGA |
Rrimp-SC-F | GGGGTACCAGGCCCACCACCCGCAGACT |
Rrimp-SC-R | GGAATTCCGGCGTGCGGCTTGGATCTA |
CrimP-SC-F | CGCGGCCAGTTCCTCACTGT |
CrimP-SC-R | CAGGGCGCTCATGTCGATGT |
ECrimP-F | TTGTCCACATTAGAGCAAAAATTAACAGAGATGATTACTGGAATTGTGAGCGGATAAC |
ECrimP-R | TCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTAGGCGATTAAGTTGGGTAA |
YZECrimP-F | TTGTCCACATTAGAGCAAA |
YZECrimP-R | TTAAAAGTGGGGAACCAG |
PFrimP-F | CGGGATCCGACCCACAACAGCACACG |
PFrimP-R | GCTCTAGATCTCCTTCTCCCGTACCAA |
LrimP-SV-F | AAGCTTGCCGAACGGTACAGAAAGGGTA |
LrimP-SV-R | TCTAGATCGCTCTGGGTGGTGCTCATC |
RrimP-SV-F | GGATCCGGCGAGTACGTCCTCGAAGT |
RrimP-SV-R | GATATCACCTTGCTCTCCACACCGAACTCC |
CrimP-SV-F | CGGCGGTTCGAAACCCATGC |
CrimP-SV-R | CTACGCCTCCTCTTCCTTCTTGTCCTT |
YZrimP-SV-F | GTGGTACGTCGTCGAAGATC |
YZrimP-SV-R | GCAAAGCGTCAGTCAACTTG |
Primers for real-time PCR of genes in S. coelicolor | Â |
RTact-F | GCTCCTCAGGCGGCACGA |
RTact-R | GCCGGCGGGTGTGGTACA |
RTred-F | GCCCTGACGCGCTATTGG |
RTred-R | GGTGGTGGGCGAGACGGA |
RTcda-F | GGAAAGCGACGCCTACTT |
RTcda-R | AGGCTCGTCTTTCCGATT |
RTmetK-F | CGAGCCCGTGGGTCTGTT |
RTmetK-R | CAGGTCGAGAGCGCGGAT |
RTsigR-F | CGACCACCTGCCCGACTC |
RTsigR-R | CCCCATGATGTCCGCGAT |
RTsigE-F | GGAGGAGGTGCCGACCGA |
RTsigE-R | TTCCCGCCGACATTCCGA |
RTsigH-F | GGAGCCGCTGGACGACCT |
RTsigH-R | CACCGCCCAGCCCTTGTC |
RTbldN-F | GACAGCGCCCGCATGATG |
RTbldN-R | GAGCGCCCGCAGAAAGGT |
RTsigT-F | GCCCTCGTCTCCGCCTAC |
RTsigT-R | CAGGCGTTCGGTGTCGTC |
RTabsR1-F | CCCGCAGTCGATCATGGA |
RTabsR1-R | GCAGGGCGAACTCCTTGTC |
RTadpA-F | AGCACCTCCACGAGCAGTTC |
RTadpA-R | CGTCCACCGAGTAGTCCGA |
RTafsR-F | GGCGGTGGATCTGCTGTG |
RTafsR-R | ACATCGCTGAGAACGGTGC |
RTatrA-F | CCGGCGGTGCGATGAGTA |
RTatrA-R | ACCCCAGCTCGCCGAACA |
RTndgR-F | CGACGTGACGGGCGAGAG |
RTndgR-R | GGAGCCGGCCTTCATGGT |
RTphoP-F | ACGTTCCCGTGATCATGGTG |
RTphoP-R | CAGTACGGCTCGGATGCG |
RTrnc-F | GGTGATCGGCGCGGTCTA |
RTrnc-R | CCTTCGGTCGCGGTGAGT |
RTssgA-F | CAGGCGCTGTTCCGTTCC |
RTssgA-R | GATGCGGTCCAGGGCCTC |
RT5072-F | GACGACCTGCCGCTCAAG |
RT5072-R | GAACGATGTGCGGTGGGT |
RT5082-F | GGAGGCCCTGGAGCAGTC |
RT5082-R | GCCGGCGATGATGATCTC |
RT5086-F | ACCTCACCGGCGTGTTCC |
RT5086-R | CGTGCTTCGAGGCGGAGT |
RT5087-F | GAACGACCGCCACGAGAC |
RT5087-R | GATCTCCAGCGAGCCGAT |
RTsanG-F | GGCCACCCTGCAGACGTAC |
RTsanG-R | CGGGACAGGTCGAACGTG |
RTpolR-F | GGTCTCCCGCGGACAACA |
RTpolR-R | GCGGCTCGTAGGACGTGA |
RThrdB-SC-F | GATCGCCGAGTCCGTCTC |
RThrdB-SC-R | CACTGAGTGGCCGGAATC |
Primers for real-time PCR of genes in S. venezuelae | Â |
RTjadR3-F | CACGTGGACGTGACGGATACGG |
RTjadR3-R | GGGTGTCGGCGAGGTTTCCTTC |
RTjadW1-F | TCGTCTGCTCCGACATCACCC |
RTjadW1-R | GCAGGAAGGAGACGCTCAGGTC |
RTjadW2-F | ACGTACTGATCCACTGCGCCTCC |
RTjadW2-R | CGATCAGGGAGTGCAGCGAGG |
RTjadW3-F | ACTACGGCAGCAACGAGAAGGC |
RTjadW3-R | AGGGCGAGGGTCATCGTGTC |
RTjadR2-F | TCGGCGATCAGTTCGGGAGC |
RTjadR2-R | AGCCATTCGCCGTTGTCCC |
RTjadR1-F | TGGACGGCTTGGAGGTCTGC |
RTjadR1-R | GGCTGCTCACATGGGTGTCG |
RTjadJ-F | CTGTCGGAGGCTCAGAACGC |
RTjadJ-R | ACGATCACGTTCGCAAGCAG |
RTjadI-F | TGCACAGCACTCTGATCGTGG |
RTjadI-R | GCGTTCGCCTCCCAGTTGTAG |
RTjadA-F | CCCCAACACCGTGGTCTCC |
RTjadA-R | GCGGTCGTTCTGCTTGGTG |
RTjadB-F | GGAGCCAGGGCAGCCAGTAC |
RTjadB-R | CGAAGGTGGAGCCGTATCCG |
RTjadC-F | GCAGCAAGACCTTCACCCTCG |
RTjadC-R | CCGACAGGTGCGCGTTGAC |
RTjadE-F | GCCGACGAGCTGTGGAACG |
RTjadE-R | GAGGCCAGGTAGCCGACGAG |
RTjadD-F | GCCTTGCTGCACGACTACCG |
RTjadD-R | ACGCCGTCCTCGTTCTCCTC |
RTjadF-F | ACGCCGCTCTGGGTGAACT |
RTjadF-R | GATGTCGAGTCCTGAGACCTTGC |
RTjadG-F | ACCTGACCGTCTTCAACCTCTTCG |
RTjadG-R | TGCTGCTGGTCCGGCTTCAC |
RTjadH-F | GACGACGACGCCGTGGAGA |
RTjadH-R | GATGTCCTCGCCCGTGATGC |
RTjadK-F | CGGCTGCGGACAGGAGTACG |
RTjadK-R | GAGGCCCAGGCTGATGTTGTG |
RTjadL-F | GGAAGGAGGAACGGAAGGACG |
RTjadL-R | ATCAGGGTGTAGAGGGCGAGG |
RTjadM-F | CCCGCTACACCGGAGTCCC |
RTjadM-R | GAGTCCCGTGCCGAGTCCC |
RTjadN-F | GCAGGGTTTCGGTCTGGAGG |
RTjadN-R | CGAGGCCGTTCTGGGTGATC |
RTjadX-F | CCACCACCGACCTCACCG |
RTjadX-R | CGAAGTGGGCGGAGGGC |
RTjadO-F | TTCCACAAGTCCAACCGCAAC |
RTjadO-R | TTCGATCAGCGGCTGGGTC |
RTjadP-F | AAGCACGTCCTGGCCGAGAAG |
RTjadP-R | GGTCCATGCCGAAGGCGATGT |
RTjadQ-F | CGACAAGCCGATGATCTACTACCC |
RTjadQ-R | GCGTGAGGTTCTTGGCGATGT |
RTjadS-F | GTCTTCCCGCCCAACCACG |
RTjadS-R | GCGAGGGAGCCAGCGTCAC |
RTjadT-F | CGACGAGGTGTACGGCACG |
RTjadT-R | CAGGATGCGTTCGGTCAGG |
RTjadU-F | CAGGTCAATCAGGTCAGCCACA |
RTjadU-R | CCGGTCGGACAGGATCAGC |
RTjadV-F | GACGAGCCGCAGGGCGAG |
RTjadV-R | CCGCTCCGCCACCATCCG |
RThrdB-SV-F | AGATTCCGCCAACCCAGTG |
RThrdB-SV-R | GAGCGTCGTCTCGTCTCGTC |
other primers | Â |
rrnFp-F | TGGAGGGAGATACGAGAACG |
rrnFp-R | CCCAGAGTGAAGGGCAGATT |
MxylE-F | TGAACCGAAGTGGATAAGTT |
MxylE-R | AGCCTTCAGATAGACACGGC |
metK-F | CGGCGGCTGGAATGAATGACCC |
metK-R | CAGGCCCGCGGCCTCGCGCA |
sigR-F | GGGCGGAGATCAGCCAGGAAAG |
sigR-R | TGACCCCGAGCCTTTCGCTTCGT |
polB-F | GGTGAAGACGCCAACGAC |
polB-R | GATCGGAGCGCGTACCAG |
sanG-F | GGGGTACCGTGCGTCAACCTCATCCCG |
sanG-R | GGAATTCGCTTGCCCGCTGGTCT |
Kan-F | TCTAGAGATCCCCTGGATACCGCTCG |
Kan-R | GGATCCGTACCCGAACCCCAGAGTC |