Table 1 Bacterial strains and plasmids used in this study
Bacterial strains/plasmids | Relevant characteristics | Source/references |
|---|---|---|
Streptomyces | Â | Â |
S. coelicolor M145 | A derivative of the wild-type strain S. coelicolor A3(2) lacking plasmids SCP1 and SCP2 | [6] |
rimP-SCDM | The rimP-SC disruption mutant of S. coelicolor | This study |
rimP-SCDMC | The complemented strain of rimP-SCDM | This study |
M145/pNIK | S. coelicolor M145 containing the entire nikkomycin biosynthetic gene cluster | This study |
rimP-SCDM/pNIK | rimP-SCDM containing the entire nikkomycin biosynthetic gene cluster | This study |
M145/pPOL | S. coelicolor M145 containing the entire polyoxin biosynthetic gene cluster | This study |
rimP-SCDM/pPOL | rimP-SCDM containing the entire polyoxin biosynthetic gene cluster | This study |
S. venezuelae ATCC10712 | The wild-type strain of S. venezuelae | [6] |
rimP-SVDM | The rimP-SV disruption mutant of S. venezuelae | This study |
rimP-SVDMC | The complemented strain of rimP-SVDM | This study |
E. coli | Â | Â |
JM109 | recA1, endA1, gyrA96, thi-1, hsdR17, supE44, relA1, Δ(lac-proAB)/F’[traD36, proAB+, lacIq, lacZΔM15] | Invitrogen |
BW25113 | K-12 derivative; ΔaraBAD ΔrhaBAD | [32] |
ET12567/pUZ8002 | dam dcm hsdS cat tet/pUZ8002 | [33] |
rimPDM | The rimP disruption mutant of E. coli BW25113 | This study |
rimPDMC | The heterologous complemented strain of rimPDM | This study |
rimPDM/pSET152::rrnFp::SCO5703 | rimPDM containing pSET152::rrnFp::SCO5703 for heterologous complementation analysis | This study |
BW25113/pSET152::rrnFp::xylE | BW25113 containing pSET152::rrnFp::xylE for catechol dioxygenase assay | This study |
BW25113/pSET152::rrnFp::xylE* | BW25113 containing pSET152::rrnFp::xylE* for catechol dioxygenase assay | This study |
rimPDM/pSET152::rrnFp::xylE | rimPDM containing pSET152::rrnFp::xylE for catechol dioxygenase assay | This study |
rimPDM/pSET152::rrnFp::xylE* | rimPDM containing pSET152::rrnFp::xylE* for catechol dioxygenase assay | This study |
Plasmids | Â | Â |
pRIMPSC3 | Plasmid used for the construction of rimP-SCDM | This study |
pRIMPSC4 | pSET152 containing the intact rimP-SC with its putative promoter | This study |
pRIMPSV3 | Plasmid used for the construction of rimP-SVDM | This study |
pRIMPSV4 | pSET152 containing the intact rimP-SV with its putative promoter | This study |
pSET152::rrnFp::xylE | pSET152 containing the wild-type xylE and the promoter of rrnF for activity detection of catechol dioxygenase | This study |
pSET152::rrnFp::xylE* | pSET152 containing the mutated xylE and the promoter of rrnF for activity detection of catechol dioxygenase | This study |
pIJ10500::metK | pIJ10500 containing the intact metK with its promoter | This study |
pIJ10500::sigR | pIJ10500 containing the intact sigR with its promoter | This study |
pNIK | pSET152 containing the entire nikkomycin biosynthetic gene cluster | [34] |
pPOL | pSET152 containing the entire polyoxin biosynthetic gene cluster | [35] |