Skip to main content

Table 2 Adaptive laboratory evolution experiments with yeasts

From: Adaptive laboratory evolution – principles and applications for biotechnology

Species, Strain

Environment

Selection time

Reference

Nutrient

S. cerevisiae

glucose-limited chemostat

250 generations

Ferea et al. 1999 [93]

S. cerevisiae

glucose- limited chemostat

500 generations

Dunham et al. 2002 [94]

S. cerevisiae capable of growth on xylose, EMS-mutagenized,

chemostat, minimal medium, anaerobic growth on xylose

170 generations

Sonderegger and Sauer 2003 [95]

S.cerevisiae

maltose-limited chemostat

> 25 generations

Jansen et al. 2004 [96]

S. cerevisiae CEN.PKPDC1,5,6

chemstat, shake flask, synthetic medium, C2-independence

nd

van Maris et al. [97]

S.cerevisiae

glucose-limited chemostat

200 generations

Jansen et al. 2005 [98]

S. cerevisiae RWB 217

chemostat, batch, xylose

Nd

Kuyper et al. 2005 [99]

S. cerevisiae

synthetic medium, arabinose

17 transfers, ca. 3500 hours

Wisselink et al. 2007 [100, 101]

S. cerevisiae

chemostat, sequential batch

40 days and 20 cycles

Wisselink et al. 2009 [102]

glucose, xylose and arabinose

S. cerevisiae DBY11331

sulfate-limited chemostat

188 generations

Araya et al. 2010 [103]

S. cerevisiae TMB3061

chemostat, synthetic medium, growth on xylose and arabinose

20 and 65 generations

Garcia Sanchez et al. [104]

S. cerevisiae CEN.JB27PYC1

Synthetic medium, selection for growth on glucose

nd

Zelle et al. 2010 [105]

S. cerevisiae CEN-PK

galactose minimal medium

400 generations

Hong et al. 2011 [106]

S. cerevisiae, engineered, scfa+, Pyc-

batch and nitrogen limited chemostat, anaerobic glucose

approx. 30 days

Zelle et al. 2011 [107]

S. cerevisiae

glucose limitation

approximately 100 generations

Wenger et al. 2011 [53]

SC288

S. cerevisiae

increased xylose fermentation

nd

Shen et al. 2012 [108]

S. cerevisiae, engineered

batch and chemostat cultures, xylose utilization and ethanol production

70 + 120 generations

Zhou et al. 2012 [109]

S. cerevisiae CMB.GS001

Increased aerobic growth on xylose

10 cycles

Scalcinati et al. 2012 [110]

S. cerevisiaejen1

synthetic medium, lactate

10 tranfers

de Kok et al. 2012 [111]

S. cerevisiae

VERT, YNB, lignocellulosic hydrolysate tolerance

463 generations

Almario et al. 2013 [112]

Environmental stress

S. cerevisiae CEN-PK EMS mutagenized

chemostat and batch selection, multiple abiotic stresses

up to 68 generations

Cakar et al. 2005 [113]

S. cerevisiae CEN-PK EMS mutagenized

YMM, continuous and pulsed CoCl 2 stress

25 transfers

Cakar et al. 2009 [114]

C. albicans

fluconazole

330 generations

Selmecki et al. 2009 [115]

S. cerevisiae BY4741

YP galactose medium, 0.5 M NaCl

300 generations

Dhar et al. 2011 [116]

S. cerevisiae BL7

YP medium, 0 – 2.5 g L-1CuSO 4

nd

Adamo et al. 2012 [117]

S. cerevisiae

SD medium, 1.17% NaCl, 37°C

25 generations

Gray and Goddard 2012 [118]

S. cerevisiae

YP medium, 6 – 8% ethanol

141 generations

Avrahami-Moyal et al. 2012 [119]

W303

S. cerevisiae

salt and oxidative stress

300 generations

Dhar et al. 2013 [120]

Miscellaneous

S. cerevisiaemyo1

cytokinesis stress

nd

Rancati et al. 2008 [54]

S.cerevisiae EC1118

SD gluconate,

240 generations

Cadière et al. 2011, 2012 [121, 122]

enological properties

  1. Experiments are presented in the approximate chronological order for the different selection conditions. The respective selection condition in the second column (environment) is highlighted in bold letters. nd - no data.