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Table 4 Plackett-Burman design represented by coded values, and proline concentration as the response

From: Ornithine cyclodeaminase-based proline production by Corynebacterium glutamicum

Run A B C D E F G H I J K L D1 D2 D3 D4 D5 D6 D7 Response
Proline (g/L)
1 - - + + + + - + - + - - - - + + + + + 4.3 ± 0.01
2 - + - - - - + + - + + - - + + + + - + 3.4 ± 0.05
3 + + + - + - + - - - - + + - + + + - + 4.1 ± 0.4
4 + - + - + - - - - + + - + + - - - + + 5.8 ± 0.2
5 + - - + + + + - + - + - - - - + + - + 7.9 ± 0.3
6 + - - - - + + - + + - - + + + + + + - 8.2 ± 0.08
7 - - - - + + - + + - - + + + + - - - + 5.3 ± 0.1
8 - - + + - + + - - + + + + - + - - - - 4.9 ± 0.01
9 + - + + - - + + + + - + - + - - - - + 6.7 ± 0.1
10 + + + + - + - + - - - - + + - + + - - 5.9 ± 0.05
11 + + - + + - - + + + + - + - + - - - - 6.8 ± 0.08
12 + + - - + + + + - + - + - - - - - + - 7.7 ± 0.4
13 - - - - - - - - - - - - - - - - - - - 6.2 ± 0
14 + + - + - + - - - - + + - + + - - + + 7.8 ± 0.4
15 - + + + + - + - + - - - - + + - - + - 3.0 ± 0.2
16 + - + - - - - + + - + + - - + + + + - 6.5 ± 0.2
17 - + + - + + - - + + + + - + - + + - - 2.5 ± 0.2
18 - + - + - - - - + + - + + - - + + + + 3.0 ± 0.2
19 - + + - - + + + + - + - + - - - - + + 2.7 ± 0.07
20 - - - + + - + + - - + + + + - + + + - 5.1 ± 0.09
  1. + indicates that the medium component was used at its high level concentration.
  2. – indicates that the medium component was used at its low level concentration.