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Table 3 Proline production with different nitrogen sources

From: Ornithine cyclodeaminase-based proline production by Corynebacterium glutamicum

Nitrogen source

Proline (g/L)

Urea, (NH4)2SO4

8.0 ± 0.2

Urea

11.5 ± 0.4

(NH4)2SO4

6.2 ± 0.1

NH4Cl

6.0 ± 0.1

  1. Titers were obtained from cells grown in CGXII medium with 40 g/L glucose, 0.75 mM arginine, 1 mM IPTG, and 25 μg/mL kanamycin.
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Microbial Cell Factories

ISSN: 1475-2859

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