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Table 3 Proline production with different nitrogen sources

From: Ornithine cyclodeaminase-based proline production by Corynebacterium glutamicum

Nitrogen source Proline (g/L)
Urea, (NH4)2SO4 8.0 ± 0.2
Urea 11.5 ± 0.4
(NH4)2SO4 6.2 ± 0.1
NH4Cl 6.0 ± 0.1
  1. Titers were obtained from cells grown in CGXII medium with 40 g/L glucose, 0.75 mM arginine, 1 mM IPTG, and 25 μg/mL kanamycin.