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Table 2 Accumulation of proline and by-products

From: Ornithine cyclodeaminase-based proline production by Corynebacterium glutamicum

 

Proline (g/L)

Ornithine (g/L)

Threonine (g/L)

Alanine (g/L)

Valine (g/L)

JJ001

< 10-4

14.8 ± 0.2

0.3 ± 0.01

0.7 ± 0.03

0.7 ± 0.01

JJ002

< 10-4

12.1 ± 0.3

0.3 ± 0.01

0.5 ± 0.01

0.5 ± 0.01

JJ003

2.4 ± 0.1

5.5 ± 0.3

0.3 ± 0.01

0.6 ± 0.01

0.4 ± 0.01

JJ004

10.0 ± 0.1

2.8 ± 0.2

0.2 ± 0.01

0.3 ± 0.01

0.3 ± 0.01

JJ004*

10.9 ± 0.3

0.2 ± 0.1

0.2 ± 0.01

0.3 ± 0.01

0.6 ± 0.01

  1. Titers were obtained from cells grown in CGXII medium with 40 g/L glucose, 0.75 mM arginine, 1 mM IPTG, and 25 μg/mL kanamycin. * Titers were obtained from cells grown in CGXIIm medium with 35 g/L glucose and supplements as stated above.