Table 3 Plasmids and strains used in this study
From: Production of hydroxycinnamoyl anthranilates from glucose in Escherichia coli
Plasmid or Strain | Description | References |
|---|---|---|
Base plasmids | ||
pZS21 | pSC101; Kanr PLtetO-1 | [61] |
pBbB5a | pBBR1; AmprlacI PlacUV5 | [62] |
pBbA5c | p15A; CmrlacI PlacUV5 | [62] |
pBbE1a | colE1; AmprlacI Ptrc | [62] |
pBbE7k | colE1; KanrlacI PT7 | [62] |
Shikimate plasmid | ||
pS0 | pZS21::ydiB-aroD-aro B-aroG*-ppsA-tktA | [45] |
Tyrosine plasmid | ||
pY | pBbB5a::tyrB-tyrA*-aroC-aroA-aroL | This study |
Cinnamoyl anthranilates plasmids | ||
pAvn | pBbA5c::HCBT-4CL1 | This study |
pAvnD | pBbA5c::HCBT-4CL1-tal | This study |
pAvnDF1 | pBbA5c::HCBT-4CL1-tal-Ptrc-sam5 | This study |
pAvnDF2 | pBbA5c::HCBT-4CL1-tal-Ptrc-hpaB-hpaC | This study |
Other plasmids | ||
pSam5 | pBbE1a::sam5 | This study |
pRgTAL | pBbE7k::tal | This study |
Strains | ||
W3110 trpD9923 | W3110 [F-λ- INV (rrnD-rrnE) 1] tryptophan auxotroph, randomly mutagenized by treatment with ultraviolet radiation | |
DH10B | Cloning host | Life technologies |
BL21(DE3) | Expression host | Life technologies |