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Table 3 Specific activity measurements from the cellodextrin transporter as determined through radioactive-labeled cellobiose uptake rate assay

From: Directed evolution of a cellobiose utilization pathway in Saccharomyces cerevisiae by simultaneously engineering multiple proteins

  Cellobiose uptake rate
(U/gcdw)
Control 0.006 ± 0.001
Wild-type 1.77 ± 0.07
D433G 2.09 ± 0.05
C82S 2.63 ± 0.2
D433G + C82S 2.82 ± 0.2
  1. One unit is defined as one μmol/min. The control is the lysate from a strain with no heterologously expressed cellodextrin transporter. Errors represent the standard deviation of biological triplicates.