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Table 3 Specific activity measurements from the cellodextrin transporter as determined through radioactive-labeled cellobiose uptake rate assay

From: Directed evolution of a cellobiose utilization pathway in Saccharomyces cerevisiae by simultaneously engineering multiple proteins

 

Cellobiose uptake rate

(U/gcdw)

Control

0.006 ± 0.001

Wild-type

1.77 ± 0.07

D433G

2.09 ± 0.05

C82S

2.63 ± 0.2

D433G + C82S

2.82 ± 0.2

  1. One unit is defined as one μmol/min. The control is the lysate from a strain with no heterologously expressed cellodextrin transporter. Errors represent the standard deviation of biological triplicates.
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Microbial Cell Factories

ISSN: 1475-2859

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