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Table 2 Specific activity measured from the crude lysate of the engineered β-glucosidase

From: Directed evolution of a cellobiose utilization pathway in Saccharomyces cerevisiae by simultaneously engineering multiple proteins

  p-NGP Cellobiose
  (U/mg protein) (U/mg protein)
Control 0.00729 ± 0.0001 0.0021 ± 0.002
Wild-type 0.193 ± 0.01 0.0668 ± 0.001
L173H 0.130 ± 0.01 0.124 ± 0.004
H23L 0.219 ± 0.004 0.0738 ± 0.006
L173H + H23L 0.156 ± 0.007 0.170 ± 0.01
  1. One unit is defined as one μmol/min and the control is the lysate from a strain with no heterologously expressed β-glucosidase. Errors are derived from the standard deviation of biological triplicates.