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Table 1 Parameters for the strains harboring the improved pathways identified through directed evolution

From: Directed evolution of a cellobiose utilization pathway in Saccharomyces cerevisiae by simultaneously engineering multiple proteins

  Wild-type R1 R2
Specific Growth Rate 0.0694 ± 0.003 0.0919 ± 0.006 0.102 ± 0.002
(h-1 )    
Cellobiose Consumption 1.78 ± 0.06 2.33 ± 0.02 2.65 ± 0.02
(g cellobiose/(L · h))    
Ethanol Productivity 0.611 ± 0.02 0.815 ± 0.03 1.00 ± 0.03
(g ethanol/(L · h))    
Yield    
(g ethanol / g cellobiose) 0.4073 ± 0.02 0.4236 ± 0.0008 0.4363 ± 0.004
  1. *Values determined at time point when >95% cellobiose was consumed for the strain.
  2. Errors are standard deviations of biological triplicates from three independent experiments. Fermentation conditions are in 8% cellobiose rich media and oxygen-limited conditions.