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Table 1 Parameters for the strains harboring the improved pathways identified through directed evolution

From: Directed evolution of a cellobiose utilization pathway in Saccharomyces cerevisiae by simultaneously engineering multiple proteins

 

Wild-type

R1

R2

Specific Growth Rate

0.0694 ± 0.003

0.0919 ± 0.006

0.102 ± 0.002

(h-1 )

   

Cellobiose Consumption

1.78 ± 0.06

2.33 ± 0.02

2.65 ± 0.02

(g cellobiose/(L · h))

   

Ethanol Productivity

0.611 ± 0.02

0.815 ± 0.03

1.00 ± 0.03

(g ethanol/(L · h))

   

Yield

   

(g ethanol / g cellobiose)

0.4073 ± 0.02

0.4236 ± 0.0008

0.4363 ± 0.004

  1. *Values determined at time point when >95% cellobiose was consumed for the strain.
  2. Errors are standard deviations of biological triplicates from three independent experiments. Fermentation conditions are in 8% cellobiose rich media and oxygen-limited conditions.