Figure 4From: Directed evolution of a cellobiose utilization pathway in Saccharomyces cerevisiae by simultaneously engineering multiple proteinsHomology model of the N. crassa β-glucosidase with the cellobiose substrate docked into the active site. (a) The full β-glucosidase homology model illustrating the L23 mutation far from the active site. (b) There are no predicted ligand interactions of the cellobiose with the wild-type L173 residue within the active site. (c) The mutated residue H173 is predicted to have a direct hydrogen bond with the hydroxyl group of the C1 atom of the cellobiose molecule.Back to article page