Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA sequences, including whole metabolic pathways, onto the Escherichia coli chromosome at well-characterised loci

Table 5 List of kiko vectors, their characteristics and genbank accession numbers

KIKO vector	Target locus	Resistance gene	GenBank accession
pKIKOarsB Cm	arsB	catP (Cm^R)	KC503965
pKIKOlacZ Cm	lacZ	catP (Cm^R)	KC503966
pKIKOrbsAR Cm	rbsAR	catP (Cm^R)	KC503967
pKIKOarsB Km	arsB	aphA (Km^R)	KC503968
pKIKOlacZ Km	lacZ	aphA (Km^R)	KC503969
pKIKOrbsAR Km	rbsAR	aphA (Km^R)	KC503970

The resistance gene listed is the gene inserted onto the chromosome after homologous recombination. Km^R kanamycin resistance, Cm^R chloramphenicol resistance.

ISSN: 1475-2859