Plasmid | Properties | Reference/ source |
---|---|---|
pKD3 | oriR6Kgamma bla catP | [23] |
pKD4 | oriR6Kgamma bla aphA | [23] |
pKD46 | repA101ts & oriR101 ParaBexo, bet, gam araC bla | [23] |
pCP20 | Flp recombinase expression plasmid | [51] |
pNPDX2 | PBTac2 (Boehringer) carrying modified xynA cDNA from Neocallimastix patriciarum | [80] |
pBAV1K-T5-gfp | pBAV1K-T5 plasmid that carries gfp gene | [81] |
pCSCX | pCR2.1 plasmid carrying cscA, cscK and cscB gene from E. coli W | [46] |
pJAS01 | Synthetic construct. Contains MCS & FRT-catP-FRT cassette flanked by hairpin loops. | This study |
parsB-R6K | JSP64/JSP65 PCR product from pKD4 (PvuII; 1,495; R6K ori and bla) ΩJSP10/JSP11 PCR product (PmeI; 1,109; arsB’) | This study |
placZ-R6K | JSP64/JSP65 PCR product from pKD4 (PvuII; 1,495; R6K ori and bla) ΩJSP57/JSP58 PCR product (PmeI; 1,318; lacZ’) | This study |
prbsAR-R6K | JSP64/JSP65 PCR product from pKD4 (PvuII; 1,495; R6K ori and bla) ΩJSP55/JSP56 PCR product (PmeI; 1,034; rbsAR’) | This study |
pKIKOarsB Cm | parsB-R6K (PmeI; 2,604; linear) ΩpJAS01 (PmeI; 1,143; MCS/catP cassette) | This study |
pKIKOlacZ Cm | placZ-R6K (PmeI; 2,813; linear) ΩpJAS01 (PmeI; 1,143; MCS/catP cassette) | This study |
pKIKOrbsAR Cm | prbsAR-R6K (PmeI; 2,529; linear) ΩpJAS01 (PmeI; 1,143; MCS/catP cassette) | This study |
pKIKOarsB Km | pKIKOarsB Cm (XbaI; 2,839; catP drop-out) ΩpKD3 (XbaI; 930; aphA) | This study |
pKIKOlacZ Km | pKIKOlacZ Cm (XbaI; 2,764; catP drop out) ΩpKD3 (XbaI; 930; aphA) | This study |
pKIKOrbsAR Km | pKIKOrbsAR Cm (XbaI; 3,111; catP drop out) ΩpKD3 (XbaI; 930; aphA) | This study |
pKIKOarsB Ωxyn Cm | pKIKOarsB Cm (PstI/SalI; 3,763) ΩpNPDX2 (PstI/SalI; 1,108; xynA) | This study |
pKIKOlacZ Ωxyn Cm | pKIKOlacZ Cm (PstI/SalI; 3,688) ΩpNPDX2 (PstI/SalI; 1,108; xynA) | This study |
pKIKOrbsAR Ωxyn Cm | pKIKOrbsAR Cm (PstI/SalI; 4,035) ΩpNPDX2 (PstI/SalI; 1,108; xynA) | This study |
pKIKOrbsAR Ωgfp Cm | pKIKOrbsAR Cm (SacI/SpeI; 4,035) ΩpBAV1K-T5-gfp (Sac I/Spe I; 1,849; aphA & gfp) | This study |
pKIKOarsB ΩcscAKB Cm | pKIKOarsB Cm (EcoRI; 3,771) ΩpCSCX (EcoRI; 4,064; cscAKB) | This study |