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Table 2 Strains used in this study

From: Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA sequences, including whole metabolic pathways, onto the Escherichia coli chromosome at well-characterised loci

Strain

Properties

Reference/source

BW23474

Δ(argF-lac)169, ΔuidA4::pir-116, recA1, rpoS396(Am), endA9(del-ins)::FRT, rph-1, hsdR514, rob-1, creC510

[78]1

MG1655

λ-, rph-1

CGSC1

MGΔarsB::xyn

MG1655 arsB::xynAcatP; CmR

This study

MGΔrbsAR::xyn

MG1655 rbsAR::xynAcatP; CmR

This study

MGΔlacZ::xyn

MG1655 lacZ::xynAcatP; CmR

This study

MGΔlacZ::xyn FRT

MG1655 lacZ::xynA-FRT; CmS

This study

MGΔlacZ::xyn ΔrbsAR::gfp-Cm

MG1655 lacZ::xynA- FRT rbsAR::gfpcatP; CmR

This study

MGΔlacZ::xyn ΔrbsAR::gfp

MG1655 ΔlacZ::xynA-FRT ΔrbsA-rbsR::gfp-FRT; CmS

This study

MGΔlacZ::xyn ΔrbsAR::gfp ΔarsB::csc-Cm

MG1655 ΔlacZ::xynA-FRT ΔrbsA-rbsR::gfp-FRT ΔarsB::cscAKBcatP; CmR

This study

MGΔlacZ::xyn ΔrbsAR::gfp ΔarsB::csc

MG1655 ΔlacZ::xynA-FRT ΔrbsA-rbsR::gfp-FRT ΔarsB::cscAKB- FRT; CmS

This study

W

Wild type strain

NCIMB 86662

WΔarsB::xyn

W arsB::xynAcatP; CmR

This study

WΔlacZ::xyn

W lacZ::xynAcatP; CmR

This study

WΔrbsAR::xyn

W rbsAR::xynAcatP; CmR

This study

  1. 1Purchased from the Coli Genetic Stock Centre (CGSC).
  2. 2National Collection of Industrial Bacteria, Aberdeen, Scotland. This strain is also archived as ATCC9637 in the American Type Culture Collection.