Figure 1From: Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA sequences, including whole metabolic pathways, onto the Escherichia coli chromosome at well-characterised lociLocation of KIKO target loci on the genome of E. coli MG1655. Map generated using the Microbial Genome Viewer (http://mgv2.cmbi.ru.nl; [47]). Locus positions relative to oriC are conserved in E. coli W. Arrows represent gene orientation.Back to article page