Table 3 Real time PCR primers for ERO1 and PDI1
From: Repressible promoters – A novel tool to generate conditional mutants in Pichia pastoris
Primer name | Primer sequence | Amplicon size (bp) |
|---|---|---|
ERO_fw: | GTTGGAAAAGCCGCATATAAACAAAACA | 141 |
ERO_rv: | CAGCTTGGGCAAAGTCCTGTAAGAGTTC | |
PDI_fw: | GGAAAGGCCCACGATGAAGTTGTC | 140 |
PDI_rv: | GCATCCTCATCATTGGCGTAAAGAGTAG | |
ACT_fw: | CCTGAGGCTTTGTTCCACCCATCT | 148 |
ACT_rv: | GGAACATAGTAGTACCACCGGACATAACGA |