Table 3 Estimated copy number (ENC) of AtGME and AtVTC genes by absolute quantification
From: Metabolic engineering of Kluyveromyces lactis for L-ascorbic acid (vitamin C) biosynthesis
Strains | CTa | Copies (Copies.μL-1) | ENC | |||||
|---|---|---|---|---|---|---|---|---|
ACT1 | GME | VTC | ACT1 | GME | VTC | GME | VTC | |
JVC1-51 | 20.43 ± 0.37 | 21.52 ± 0.43 | 20.19 ± 1.23 | 2.95E + 04 | 4.35E + 03 | 1.61E + 04 | 1 | 1 |
JVC1-53 | 18.92 ± 0.61 | 19.52 ± 1.69 | 20.01 ± 0.08 | 1.34E + 04 | 4.26E + 04 | 1.14E + 04 | 3 | 1 |
JVC1-56 | 15.88 ± 2.28 | 15.43 ± 198 | 16.72 ± 0.32 | 1.06E + 05 | 4.02E + 05 | 1.70E + 05 | 4 | 2 |
JVC3-18 | 18.68 ± 0.35 | 20.41 ± 0.17 | 14.88 ± 0.95 | 8.21E + 04 | 1.58E + 04 | 2.98E + 05 | 1 | 4 |