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Table 1 Yeasts strains used in this study

From: Metabolic engineering of Kluyveromyces lactis for L-ascorbic acid (vitamin C) biosynthesis

Strains Markers Cassette expression Plasmids Reference
CBS 2359 Wild type - - Genolevures consortium*
JVC1-5 KanR AtGME pKLJC/GME This study
JVC1-51 KanR, amDs AtGME, AtVTC2, AtVTC4 pKLJC/GME, pKlVTc This study
JVC1-53 KanR, amDs AtGME, AtVTC2, AtVTC4 pKLJC/GME, pKlVTc This study
JVC1-56 KanR, amDs AtGME, AtVTC2, AtVTC4 pKLJC/GME, pKlVTc This study
JVC2-1 amDs AtVTC2, AtVTC4 pKlVTc This study
JVC2-2 amDs AtVTC2, AtVTC4 pKlVTc This study
JVC3-18 KanR, amDs AtGME, AtVTC2, AtVTC4 pKLJC/GME, pKlVTc This study
  1. KanR cassette conferring resistance to Geneticin.
  2. amDs acetamidase marker.
  3. *Kluyveromyces lactis strain used for Genome sequencing by the Génolures consortium (http://www.genolevures.org).