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Figure 3 | Microbial Cell Factories

Figure 3

From: Metabolic engineering of Kluyveromyces lactis for L-ascorbic acid (vitamin C) biosynthesis

Figure 3

Expression analyses of L-AA pathway plant genes by recombinant K. lactis cells. (A) RT-PCR using cDNA from K. lactis cells transformed with three early L-AA pathway plant genes from Arabidopsis thaliana. C + − the cDNA from A. thaliana leaves and plasmids harboring the corresponding genes were used as controls for RT-PCR analysis. KlACT1 gene from K. lactis CBS2359 was used as a control for RNA quality. (B) – Western blotting of flag-tagged immunoprecipitated proteins from K. lactis recombinant cells using monoclonal anti-flag antibody. K. lactis CBS2359 strain was used as negative control. The RNA extraction and total protein extraction were carried out from cells grown in YP medium with 2% (w/v) D-Galactose after 24 hours incubation at 30°C, 200 rpm.

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