Production of l-lactic acid by the yeast Candida sonorensis expressing heterologous bacterial and fungal lactate dehydrogenases

Table 3 Plasmids for C. sonorensis transformations constructed in this work

Plasmid	Relevant content
pMI234	CsP _PGK1 -ScMEL5
pMI238	CsP _TDH1- ScMEL5
pMI246	CsP _PGK1 -ScMEL5-ScT _MEL5 - CsP _PGK1 -LhLDH-ScT _CYC1
pMI247	CsP _GPD1 -ScMEL5-ScT _MEL5 - CsP _PGK1 -LhLDH-ScT _CYC1
pMI257	CsPDC1 5′ - CsP _PGK1 -ScMEL5-ScT _MEL5 - CsP _PGK1 -LhLDH-ScT _CYC1 - CsPDC1 3′
pMI265	CsPDC1 5′ - CsP _PGK1 -ScMEL5-ScT _MEL5 - CsP _PGK1 -BmLDH-CsPDC1 3′
pMI266	CsPDC1 5′ - CsP _PGK1 -ScMEL5-ScT _MEL5 - CsP _PGK1 -RoLDH-CsPDC1 3′
pMI267	CsPDC1 5′ - CsP _PGK1 -ScMEL5-ScT _MEL5 - CsP _PGK1 -CsPDC1 3′
pMI268	CsP _PGK1 1-G418 ^R -ScT _GAL10
pMI269	CsP _GPD1 -G418 ^R -ScT _GAL10
pMI278	CsP _GPD1 -G418 ^R -ScT _MEL5 - CsP _PGK1 -BmLDH-ScT _GAL10
pMI279	CsPDC2 5′ - CsP_GPD1-G418^R-ScT_MEL5- CsP_PGK1-BmLDH-ScT_GAL10
pMI286	CsPDC2 5′ - CsP _GPD1 -G418 ^R -ScT _MEL5 - CsP _PGK1 -BmLDH-ScT _GAL10 - CsPDC2 3′
pMI287	CsPDC2 5′- CsP _GPD1 -G418 ^R -ScT _MEL5 - CsP _PGK1- CsPDC2 3′
pMI288	CsPDC2 5′- CsP _GPD1 - G418 ^R -ScT _MEL5 - CsP _PGK1 -LhLDH-ScT _CYC1 - CsPDC2 3′

ISSN: 1475-2859