Table 3 Primers used in this study

To add SHR-sequences

E.coli Rv +A

HPLC

GTGCCTATTGATGATCTGGCGGAATGTCTGCCGTGCCATAGCCATGCCTTCACATATAGTTGCGCGGAACCCCTATTTG

E.coli Fw +I

HPLC

TATTCACGTAGACGGATAGGTATAGCCAGACATCAGCAGCATACTTCGGGAACCGTAGGCGAGAGGCGGTTTGCGTATTGG

TPI1 Rv +H

HPLC

AGATTACTCTAACGCCTCAGCCATCATCGGTAATAGCTCGAATTGCTGAGAACCCGTGACTAGTGTGAGCGGGATTTAAACTGTG

TPI1 Fw +I

HPLC

GCCTACGGTTCCCGAAGTATGCTGCTGATGTCTGGCTATACCTATCCGTCTACGTGAATAGCGAAAATGACGCTTGCAGTG

FBA1 Rv +H

HPLC

GTCACGGGTTCTCAGCAATTCGAGCTATTACCGATGATGGCTGAGGCGTTAGAGTAATCTAAAATCTCAAAAATGTGTGGGTCATTACG

FBA1 Fw +G

HPLC

GCCAGAGGTATAGACATAGCCAGACCTACCTAATTGGTGCATCAGGTGGTCATGGCCCTTAGTGCATGACAAAAGATGAGCTAGG

Cen6 Ars4 Rv +G

HPLC

AAGGGCCATGACCACCTGATGCACCAATTAGGTAGGTCTGGCTATGTCTATACCTCTGGCGACGGATCGCTTGCCTGTAAC

Cen6 Ars4 Fw +F

HPLC

CATACGTTGAAACTACGGCAAAGGATTGGTCAGATCGCTTCATACAGGGAAAGTTCGGCAGTGCCACCTGGGTCCTTTTC

Cen6 Ars4 Rv +B

HPLC

CACCTTTCGAGAGGACGATGCCCGTGTCTAAATGATTCGACCAGCCTAAGAATGTTCAACGTGCCACCTGGGTCCTTTTC

Cen6 Ars4 Fw +C

HPLC

CTAGCGTGTCCTCGCATAGTTCTTAGATTGTCGCTACGGCATATACGATCCGTGAGACGTGACGGATCGCTTGCCTGTAAC

PFK2 Rv +F

HPLC

TGCCGAACTTTCCCTGTATGAAGCGATCTGACCAATCCTTTGCCGTAGTTTCAACGTATGATAGCCATTCTCTGCTGCTTTGTTG

PFK2 Fw +J

HPLC

GGCCGTCATATACGCGAAGATGTCCAAGCAGGTAGAACACATAGTCTGAGCATCTCGTCGGAGATCCGAGGGACGTTTATTGG

PFK1 Rv +D

HPLC

ACGCATCTACGACTGTGGGTCCCGTGGAGAAATGTATGAAACCCTGTATGGAGAGTGATTTCGAGATTCCTCAATCCATACACCATTATAG

PFK1 Fw +J

HPLC

CGACGAGATGCTCAGACTATGTGTTCTACCTGCTTGGACATCTTCGCGTATATGACGGCCTGTCGTCTTCGTGAACCATTGTC

PGI1 Rv +D

HPLC

AATCACTCTCCATACAGGGTTTCATACATTTCTCCACGGGACCCACAGTCGTAGATGCGTCTGAAGAAGGCATACTACGCCAAG

PGI1 Fw +C

HPLC

ACGTCTCACGGATCGTATATGCCGTAGCGACAATCTAAGAACTATGCGAGGACACGCTAGTTCGCGACACAATAAAGTCTTCACG

HXK2 Rv +C

HPLC

CTAGCGTGTCCTCGCATAGTTCTTAGATTGTCGCTACGGCATATACGATCCGTGAGACGTGCAAGAGAAAAAAACGAGCAATTGTTAAAAG

HXK2 Fw +B

HPLC

CACCTTTCGAGAGGACGATGCCCGTGTCTAAATGATTCGACCAGCCTAAGAATGTTCAACGACGGCACCGGGAAATAAACC

URA3K.l. Rv +B

HPLC

GTTGAACATTCTTAGGCTGGTCGAATCATTTAGACACGGGCATCGTCCTCTCGAAAGGTGCTCAGAAGCTCATCGAACTGTCATC

URA3K.l. Fw + A

HPLC

ACTATATGTGAAGGCATGGCTATGGCACGGCAGACATTCCGCCAGATCATCAATAGGCACGATCCCAATACAACAGATCACGTGATC

FUS1

HPLC

ACTATATGTGAAGGCATGGCTATGG

FUS2

HPLC

CTAGCGTGTCCTCGCATAGTTC

Amp-rv + A-ctrl

HPLC

TCTAGACCTAATAACTTCGTATAGCATACATTATACGAAGTTATATTAAGGGTTGTCGACTGCGCGGAACCCCTATTTG

Amp-fw + I-ctrl

HPLC

TTGGCAATTTTTTGCTCTTCTATATAACAGTTGAAATTTGAATAAGAACATCTTCTCAAAGAGAGGCGGTTTGCGTATTGG

CEN/6ARS4-fw + F-ctrl

HPLC

GAATAGTCTTTACACCCACAGTTTTTCGTGTGGCAGTTACTATATATTAGTAGGATATTCGTGCCACCTGGGTCCTTTTC

CEN6ARS4-rv + G-ctrl

HPLC

AAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGGACGGATCGCTTGCCTGTAAC

FBA1-fw + G-ctrl

HPLC

CTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTAGTGCATGACAAAAGATGAGCTAGG

FBA1-rv + H-ctrl

HPLC

TTACGGATATTTAACTTACTTAGAATAATGCCATTTTTTTGAGTTATAATAATCCTACGTAAAATCTCAAAAATGTGTGGGTCATTACG

HXK2-fw + B-ctrl

HPLC

GAGTGTTTAGAACATAATCAGTTTATCCATGGTCTATCTCTTCTTGTCGCTTTTTCTCCTGACGGCACCGGGAAATAAACC

HXK2-rv + C-ctrl

HPLC

AACAATAAATACGAAATCCTTATATAAGCATCTTTTACTACCAAAAAAATTTAAAATTAAGCAAGAGAAAAAAACGAGCAATTGTTAAAAG

K.l.URA3 -fw + A-ctrl

HPLC

GTCGACAACCCTTAATATAACTTCGTATAATGTATGCTATACGAAGTTATTAGGTCTAGAGATCCCAATACAACAGATCACGTGATC

K.l.URA3-rv + B-ctrl

HPLC

AGGAGAAAAAGCGACAAGAAGAGATAGACCATGGATAAACTGATTATGTTCTAAACACTCCTCAGAAGCTCATCGAACTGTCATC

PFK1-fw + J-ctrl

HPLC

ATATTTATAAAGGTACCCTTTTAATACAATATTTATACATGTGCGTTAAATACTTTGTTCTGTCGTCTTCGTGAACCATTGTC

PFK1-rv + D-ctrl

HPLC

TAATATTTTTTCTTTTGAAAGTACTACCCACATCCGAACATTGCCACTTACATAGCGATGTCGAGATTCCTCAATCCATACACCATTATAG

PFK2-fw + J-ctrl

HPLC

GAACAAAGTATTTAACGCACATGTATAAATATTGTATTAAAAGGGTACCTTTATAAATATGAGATCCGAGGGACGTTTATTGG

PFK2-rv + F-ctrl

HPLC

GAATATCCTACTAATATATAGTAACTGCCACACGAAAAACTGTGGGTGTAAAGACTATTCATAGCCATTCTCTGCTGCTTTGTTG

PGI-fw + C-ctrl

HPLC

TTAATTTTAAATTTTTTTGGTAGTAAAAGATGCTTATATAAGGATTTCGTATTTATTGTTTTCGCGACACAATAAAGTCTTCACG

PGI-rv + D-ctrl

HPLC

CATCGCTATGTAAGTGGCAATGTTCGGATGTGGGTAGTACTTTCAAAAGAAAAAATATTACTGAAGAAGGCATACTACGCCAAG

TPI-fw + I-ctrl

HPLC

TTTGAGAAGATGTTCTTATTCAAATTTCAACTGTTATATAGAAGAGCAAAAAATTGCCAAGCGAAAATGACGCTTGCAGTG

TPI-rv + H-ctrl

HPLC

ACGTAGGATTATTATAACTCAAAAAAATGGCATTATTCTAAGTAAGTTAAATATCCGTAATAGTGTGAGCGGGATTTAAACTGTG

For multipex PCR

A Ctrl Fw

Desalted

AAATAAACAAATAGGGGTTCCGC

A Ctrl Rv

Desalted

GCAACACTCACTTCAACTTCATC

B Ctrl Fw

Desalted

TTACCACCATCCAATGCAGAC

B Ctrl Rv

Desalted

ACGGAATAGAACACGATATTTGC

C Ctrl Fw

Desalted

TCACGGGATTTATTCGTGACG

C Ctrl Rv

Desalted

GCGTCCAAGTAACTACATTATGTG

D Ctrl Fw

Desalted

ACTCGCCTCTAACCCCACG

D Ctrl Rv

Desalted

ACGGACTATAATGGTGTATGGATTG

J Ctrl Fw

Desalted

GCTTAATCTGCGTTGACAATGG

J Ctrl Rv

Desalted

CAATAAACGTCCCTCGGATCTC

F Ctrl Fw

Desalted

GACGCCATTTGGAACGAAAAAAAG

F Ctrl Rv

Desalted

ATAGCACGTGATGAAAAGGAC

G Ctrl Fw

Desalted

GCGTGTAAGTTACAGGCAAGC

G Ctrl Rv

Desalted

GCTCTTTTCTTCTGAAGGTCAATG

H Ctrl Fw

Desalted

GTTACGTGCTCAGTTGTTAGATATG

H Ctrl Rv

Desalted

GCAGAAGTGTCTGAATGTATTAAGG

I Ctrl Fw

Desalted

TGAGCCACTTAAATTTCGTGAATG

I Ctrl Rv

Desalted

GCCTTTGAGTGAGCTGATACC