Table 2 Strains used in this study

CEN.PK113-7D

MATα MAL2-8c SUC2

[26, 33]

CEN.PK113-5D

MATα ura3-52 MAL2-8c SUC2

[33]

IMX188

MATa ura3-52 his3- Δ1 leu2-3,112 MAL2-8c SUC2 glk1 Δ::LoxP hxk1 Δ::LoxP hxk2 Δ::LoxP-KanMX-LoxP

This study

IMX214

MATa ura3-52 his3- Δ1 leu2-3,112 MAL2-8c SUC2 glk1 Δ::LoxP hxk1 Δ::LoxP hxk2 Δ::LoxP-KanMX-LoxP + PUDC074

This study

BY4743-Y23336

MATa/α; ura3 Δ0/ura3 Δ0; pgi1 Δ::KanMX/ PGI1

EUROSCARF^a

BY4743-Y25893

MATa/α; ura3 Δ0/ ura3 Δ0; pfk1 Δ:: KanMX/PFK1

EUROSCARF^a

BY4743-Y20791

MATa/α; ura3 Δ0/ura3 Δ0; pfk2 Δ::KanMX/PFK2

EUROSCARF^a

BY4743-Y23986

MATa/α; ura3 Δ0/ura3 Δ0; tpi1 Δ::KanMX/TPI1

EUROSCARF^a

BY4743-Y24909

MATa/α; ura3 Δ0/ura3 Δ0; fba1 Δ::KanMX/FBA1

EUROSCARF^a

Spore from Y23336 + pUDC074

ura3 Δ0; pgi1::kanMX pUDC074 (K.I.URA3(pUG72)TPI HXK2 FBA1 PGI PFK1 PFK2)

This study

Spore from Y25893 + pUDC074

ura3 Δ0; pfk1::kanMX pUDC074 (K.I.URA3(pUG72)TPI HXK2 FBA1 PGI PFK1 PFK2)

This study

Spore from Y20791 + pUDC074

ura3 Δ0; pfk2::kanMX pUDC074 (K.I.URA3(pUG72)TPI HXK2 FBA1 PGI PFK1 PFK2)

This study

Spore from Y24909 + pUDC074

ura3 Δ0; tpi1::kanMX pUDC074 (K.I.URA3(pUG72)TPI HXK2 FBA1 PGI PFK1 PFK2)

This study

Spore from Y23986 + pUDC074

ura3 Δ0; fba1::kanMX pUDC074 (K.I.URA3(pUG72)TPI HXK2 FBA1 PGI PFK1 PFK2)

This study