Figure 1

Influence of the plasmid backbone structure on the in vivo assembly efficiency. The quantification of the number of transformants obtained after transformation of 100 fmol of each of the corresponding fragment sets was based on triplicate experiments and the data presented are average ± standard deviation. (#) Transformation of the pRS416 backbone linearized by BamHI (1^st bar) gave so many transformants that the exact number of transformants could not be determined due to the colony density on the plates, but it exceeded 1000 transformants in all three transformations. (*) Transformation of the K. lactis URA3 fragment only (rightmost bar) did not yield any transformants.